High-throughput quantitative imaging of cell spreading dynamics by multi-step microscopy projection photolithography based on a cell-friendly photoresist.

نویسندگان

  • Jong-Cheol Choi
  • Junsang Doh
چکیده

A new method for the high-throughput study of cell spreading dynamics is devised by multi-step microscopy projection photolithography based on a cell-friendly photoresist. By releasing a large number of rounded cells in single cell arrays and monitoring their spreading dynamics by interference reflection microscopy, a large number of cell spreading data can be acquired by a single experiment.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Microfabrication of Thin-Film Solar Cell Contacts Through Photolithography

Solar energy has emerged as an inexpensive and powerful means for clean energy production and draws much research interest. In particular, thinfilm solar cells often rely on the use of permanent shadow masks to pattern their front metal contacts for energy transport. However, these machined masks are not adjustable, and currently limit the scope of experimentation. In addition, the essence of f...

متن کامل

Maskless liquid-crystal-display projection photolithography for improved design flexibility of cellular micropatterns.

We previously developed an all-in-one photopolymerization device by modifying a commercially available liquid crystal display projector (LCDP) for the preparation of micropatterned surfaces and microfluidic channels without the need for expensive photomasks. In the present study, we demonstrate a simple and reliable method for rapid prototyping of cell micropatterning with high resolution using...

متن کامل

3D Microfabrication Using Emulsion Mask Grayscale Photolithography Technique

Recently, the rapid development of technology such as biochips, microfluidic, micro-optical devices and micro-electromechanical-systems (MEMS) demands the capability to create complex design of three-dimensional (3D) microstructures. In order to create 3D microstructures, the traditional photolithography process often requires multiple photomasks to form 3D pattern from several stacked photores...

متن کامل

Use of photolithography to encode cell adhesive domains into protein microarrays.

Protein microarrays are rapidly emerging as valuable tools in creating combinatorial cell culture systems where inducers of cellular differentiation can be identified in a rapid and multiplexed fashion. In the present study, protein microarraying was combined with photoresist lithography to enable printing of extracellular matrix (ECM) protein arrays while precisely controlling "on-the-spot" ce...

متن کامل

Dry Photolithographic Patterning Process for Organic Electronic Devices using Supercritical Carbon Dioxide

The particular challenge of micro-patterning organic materials has stimulated numerous approaches for making effective and repeatable patterned structures with fine features. Among all the micro-patterning techniques photolithography, being the preferred method for the inorganic semiconductor industry, did not create much impact due to its incompatibility with the majority of organic electronic...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Lab on a chip

دوره 12 23  شماره 

صفحات  -

تاریخ انتشار 2012